Jesper Troelsen
Professor
Curriculum Vitae
Jesper Troelsen
Professor
- The Department of Science, Systems and Models
- Eukaryot Cellebiologi
Universitetsvej 1, 17.2
DK-4000, Roskilde
Denmark
E-mail: troelsen@ruc.dk
Phone: 4674-2728
Website: http://www.ruc.dk/~troelsen
Degrees
Master degree (Cand. scient.) in biology (Molecular Biology) from The Department for Microbiology, Faculty of Science, University of Copenhagen
PhD, Department for Medical Biochemistry and Genetics (IMBG), The Faculty of Health Science, University of Copenhagen.
Doctor in Medical Sciences (dr.med.), Department for Cellular Molecular Medicine (ICMM), The Faculty of Health Science, University of Copenhagen.
PhD, Department for Medical Biochemistry and Genetics (IMBG), The Faculty of Health Science, University of Copenhagen.
Doctor in Medical Sciences (dr.med.), Department for Cellular Molecular Medicine (ICMM), The Faculty of Health Science, University of Copenhagen.
Positions
Research assistant. University of Copenhagen.1991-1992
PhD-student (Kandidatstipendiat). University of Copenhagen.1992-1995
Assistant professor at the Department for Medical Biochemistry and Genetics. University of Copenhagen. 1995-1999
Weimann-sponsored research associated professor at the Department for Medical Biochemistry and Genetics1999-2000
Associate professor at the Department for Medical Biochemistry and Genetics. University of Copenhagen.2000-2006
Associate professor, Department of Cellular & Molecular Medicine, University of Copenhagen.2007-2011
Professor mso in medicinalbiology, Department for Science, Models and Systems (NSM), Roskilde University. 2011-
PhD-student (Kandidatstipendiat). University of Copenhagen.1992-1995
Assistant professor at the Department for Medical Biochemistry and Genetics. University of Copenhagen. 1995-1999
Weimann-sponsored research associated professor at the Department for Medical Biochemistry and Genetics1999-2000
Associate professor at the Department for Medical Biochemistry and Genetics. University of Copenhagen.2000-2006
Associate professor, Department of Cellular & Molecular Medicine, University of Copenhagen.2007-2011
Professor mso in medicinalbiology, Department for Science, Models and Systems (NSM), Roskilde University. 2011-
Publications
Regulation of APC and AXIN2 expression by intestinal tumor suppressor CDX2 in colon cancer cells.
Olsen, A. K., Coskun, M., Bzorek, M., Kristensen, M., Danielsen, E. T., Jørgensen, S., Olsen, J., Engel, U., Troelsen, J. & Holck, S. 2013 In : Carcinogenesis.Publication: Research - peer-review › Journal article
Current and emerging approaches to define intestinal epithelium-specific transcriptional networks
Olsen, A. K., Boyd, M., Danielsen, E. T. & Troelsen, J. 2012 In : American Journal of Physiology: Gastrointestinal and Liver Physiology. 302, 3, p. G277-86Publication: Research - peer-review › Review
pcaGoPromoter - An R Package for Biological and Regulatory Interpretation of Principal Components in Genome-Wide Gene Expression Data
Hansen, M., Gerds, T. A., Sedelin, J. B., Troelsen, J. & Olsen, J. 2012 In : P L o S One. 7, 2, p. e32394Publication: Research - peer-review › Journal article
TNF-α-induced down-regulation of CDX2 suppresses MEP1A expression in colitis
Coskun, M., Olsen, A. K., Holm, T. L., Kvist, P. H., Nielsen, O. H., Riis, L. B., Olsen, J. & Troelsen, J. 2012 In : B B A - Molecular Basis of Disease. 1822, 6, p. 843-851 9 p.Publication: Research - peer-review › Journal article
The −14010*C variant associated with lactase persistence is located between an Oct-1 and HNF1α binding site and increases lactase promoter activity
Jensen, T. G. K., Liebert, A., Lewinsky, R., Swallow, D. M., Olsen, J. & Troelsen, J. 31 Dec 2011 In : Human Genetics. 130, 4, p. 483-493Publication: Research - peer-review › Journal article
Previous publications
1. Coskun M, Troelsen JT, Nielsen OH: The role of CDX2 in intestinal homeostasis and inflammation. Biochim Biophys Acta 2011, 1812(3):283-289.
2. Maltesen HR, Troelsen JT, Olsen J: Identification of a functional hepatocyte nuclear factor 4 binding site in the neutral ceramidase promoter. J Cell Biochem 2010, 111(5):1330-1336.
3. Farooq M, Troelsen JT, Boyd M, Eiberg H, Hansen L, Hussain MS, Rehman S, Azhar A, Ali A, Bakhtiar SM et al: Preaxial polydactyly/triphalangeal thumb is associated with changed transcription factor-binding affinity in a family with a novel point mutation in the long-range cis-regulatory element ZRS. Eur J Hum Genet 2010, 18(6):733-736.
4. Coskun M, Boyd M, Olsen J, Troelsen JT: Control of intestinal promoter activity of the cellular migratory regulator gene ELMO3 by CDX2 and SP1. J Cell Biochem 2010, 109(6):1118-1128.
5. Boyd M, Hansen M, Jensen TG, Perearnau A, Olsen AK, Bram LL, Bak M, Tommerup N, Olsen J, Troelsen JT: Genome-wide analysis of CDX2 binding in intestinal epithelial cells (Caco-2). J Biol Chem 2010, 285(33):25115-25125.
6. Olsen J, Gerds TA, Seidelin JB, Csillag C, Bjerrum JT, Troelsen JT, Nielsen OH: Diagnosis of ulcerative colitis before onset of inflammation by multivariate modeling of genome-wide gene expression data. Inflamm Bowel Dis 2009, 15(7):1032-1038.
7. Broom OJ, Widjaya B, Troelsen J, Olsen J, Nielsen OH: Mitogen activated protein kinases: a role in inflammatory bowel disease? Clin Exp Immunol 2009, 158(3):272-280.
8. Boyd M, Bressendorf S, Møller J, Olsen J, Troelsen JT: Mapping of HNF4α target genes in intestinal epithelial cells. BMC Gastroenterology 2009, 9(1):68.
9. Vestergaard EM, Troelsen J, Lange A: [Lactose intolerance. Genotyping simplifies and improves diagnosis]. Ugeskr Laeger 2008, 170(42):3312-3314.
10. Schjoldager KT, Maltesen HR, Balmer S, Lund LR, Claesson MH, Sjostrom H, Troelsen JT, Olsen J: Cellular cross talk in the small intestinal mucosa: postnatal lymphocytic immigration elicits a specific epithelial transcriptional response. Am J Physiol Gastrointest Liver Physiol 2008, 294(6):G1335-1343.
11. Enattah NS, Jensen TG, Nielsen M, Lewinski R, Kuokkanen M, Rasinpera H, El-Shanti H, Seo JK, Alifrangis M, Khalil IF et al: Independent Introduction of Two Lactase-Persistence Alleles into Human Populations Reflects Different History of Adaptation to Milk Culture. Am J Hum Genet 2008, 82(1):57-72.
12. Eiberg H, Troelsen J, Nielsen M, Mikkelsen A, Mengel-From J, Kjaer KW, Hansen L: Blue eye color in humans may be caused by a perfectly associated founder mutation in a regulatory element located within the HERC2 gene inhibiting OCA2 expression. Hum Genet 2008, 123(2):177-187.
13. Stegmann A, Hansen M, Wang Y, Larsen JB, Lund LR, Ritie L, Nicholson JK, Quistorff B, Simon-Assmann P, Troelsen JT et al: Metabolome, transcriptome and bioinformatic cis-element analyses point to HNF-4 as a central regulator of gene expression during enterocyte differentiation. Physiol Genomics 2006, 11(2):141-155.
14. Troelsen JT: Adult-type hypolactasia and regulation of lactase expression. Biochimica et Biophysica Acta 2005, 1723(1-3):19-32.
15. Olsen L, Bressendorff S, Troelsen JT, Olsen J: Differentiation-dependent activation of the human intestinal alkaline phosphatase promoter by HNF-4 in intestinal cells. Am J Physiol Gastrointest Liver Physiol 2005, 289(2):G220-G226.
16. Lewinsky RH, Jensen TG, Moller J, Stensballe A, Olsen J, Troelsen JT: T-13910 DNA variant associated with lactase persistence interacts with Oct-1 and stimulates lactase promoter activity in vitro. Hum Mol Genet 2005, 14(24):3945-3953.
17. Olsen L, Hansen M, Ekstrom CT, Troelsen JT, Olsen J: CVD: the intestinal crypt/villus in situ hybridization database. Bioinformatics 2004, 20(8):1327-1328.
18. Troelsen JT, Olsen J, Moller J, Sjostrom H: An upstream polymorphism associated with lactase persistence has increased enhancer activity. Gastroenterology 2003, 125(6):1686-1694.
19. Troelsen JT, Mitchelmore C, Olsen J: An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Gene 2003, 305:101-111.
20. Olsen J, Kirkeby LT, Brorsson MM, Dabelsteen S, Troelsen JT, Bordoy R, Fenger K, Larsson LI, Simon-Assmann P: Converging signals synergistically activate the LAMC2 promoter and lead to accumulation of the laminin gamma 2 chain in human colon carcinoma cells. Biochemical Journal 2003, 371(Pt 1):211-221.
21. Dabelsteen S, Troelsen JT, Olsen J: Identification of keratinocyte proteins that mark subsets of cells in the epidermal stratum basale: comparisons with the intestinal epithelium. OncolRes 2003, 13(6-10):393-398.
22. Tadjali M, Seidelin JB, Olsen J, Troelsen JT: Transcriptome changes during intestinal cell differentiation. Biochimica et Biophysica Acta 2002, 1589(2):160-167.
23. Brimnes MK, Jensen T, Jorgensen TN, Michelsen BK, Troelsen J, Werdelin O: Low expression of insulin in the thymus of non-obese diabetic mice. J Autoimmun 2002, 19(4):203-213.
24. Olsen J, Lefebvre O, Fritsch C, Troelsen JT, Orian-Rousseau V, Kedinger M, Simon-Assmann P: Involvement of activator protein 1 complexes in the epithelium-specific activation of the laminin gamma2-chain gene promoter by hepatocyte growth factor (scatter factor). Biochem J 2000, 347(Pt 2):407-417.
25. Mitchelmore C, Troelsen JT, Spodsberg N, Sjöström H, Norén O: Interaction between the homeodomain proteins Cdx2 and HNF1alpha mediates expression of the lactase-phlorizin hydrolase gene. Biochem J 2000, 346 Pt 2:529-535.
26. Spodsberg N, Troelsen JT, Carlsson P, Enerback S, Sjöström H, Norén O: Transcriptional regulation of pig lactase-phlorizin hydrolase: involvement of HNF-1 and FREACs. Gastroenterology 1999, 116(4):842-854.
27. Mitchelmore C, Troelsen JT, Sjöström H, Norén O: The HOXC11 homeodomain protein interacts with the lactase-phlorizin hydrolase promoter and stimulates HNF1alpha-dependent transcription. Journal of Biological Chemistry 1998, 273(21):13297-13306.
28. Troelsen JT, Mitchelmore C, Spodsberg N, Jensen AM, Noren O, Sjostrom H: Regulation of lactase-phlorizin hydrolase gene expression by the caudal-related homoeodomain protein Cdx-2. Biochem J 1997, 322 ( Pt 3):833-838.
29. Olsen J, Kokholm K, Troelsen JT, Laustsen L: An enhancer with cell-type dependent activity is located between the myeloid and epithelial aminopeptidase N (CD 13) promoters. Biochemical Journal 1997, 322:899-908.
30. Thomsen PD, Johansson M, Troelsen JT, Andersson L: The lactase phlorizin hydrolase (LCT) gene maps to pig chromosome 15q13. Anim Genet 1995, 26(1):49-52.
31. Troelsen JT, Olsen J, Mitchelmore C, Hansen GH, Sjostrom H, Noren O: Two intestinal specific nuclear factors binding to the lactase-phlorizin hydrolase and sucrase-isomaltase promoters are functionally related oligomeric molecules. FEBS Lett 1994, 342(3):297-301.
32. Troelsen JT, Mehlum A, Olsen J, Spodsberg N, Hansen GH, Prydz H, Noren O, Sjostrom H: 1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice. FEBS Lett 1994, 342(3):291-296.
33. Olsen J, Laustsen L, Troelsen J: HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells. FEBS Lett 1994, 342(3):325-328.
34. Torp N, Rossi M, Troelsen JT, Olsen J, Danielsen EM: Lactase-phlorizin hydrolase and aminopeptidase N are differentially regulated in the small intestine of the pig. Biochemical Journal 1993, 295:177-182.
35. Troelsen JT, Olsen J, Noren O, Sjostrom H: A novel intestinal trans-factor (NF-LPH1) interacts with the lactase-phlorizin hydrolase promoter and co-varies with the enzymatic activity. J Biol Chem 1992, 267(28):20407-20411.
2. Maltesen HR, Troelsen JT, Olsen J: Identification of a functional hepatocyte nuclear factor 4 binding site in the neutral ceramidase promoter. J Cell Biochem 2010, 111(5):1330-1336.
3. Farooq M, Troelsen JT, Boyd M, Eiberg H, Hansen L, Hussain MS, Rehman S, Azhar A, Ali A, Bakhtiar SM et al: Preaxial polydactyly/triphalangeal thumb is associated with changed transcription factor-binding affinity in a family with a novel point mutation in the long-range cis-regulatory element ZRS. Eur J Hum Genet 2010, 18(6):733-736.
4. Coskun M, Boyd M, Olsen J, Troelsen JT: Control of intestinal promoter activity of the cellular migratory regulator gene ELMO3 by CDX2 and SP1. J Cell Biochem 2010, 109(6):1118-1128.
5. Boyd M, Hansen M, Jensen TG, Perearnau A, Olsen AK, Bram LL, Bak M, Tommerup N, Olsen J, Troelsen JT: Genome-wide analysis of CDX2 binding in intestinal epithelial cells (Caco-2). J Biol Chem 2010, 285(33):25115-25125.
6. Olsen J, Gerds TA, Seidelin JB, Csillag C, Bjerrum JT, Troelsen JT, Nielsen OH: Diagnosis of ulcerative colitis before onset of inflammation by multivariate modeling of genome-wide gene expression data. Inflamm Bowel Dis 2009, 15(7):1032-1038.
7. Broom OJ, Widjaya B, Troelsen J, Olsen J, Nielsen OH: Mitogen activated protein kinases: a role in inflammatory bowel disease? Clin Exp Immunol 2009, 158(3):272-280.
8. Boyd M, Bressendorf S, Møller J, Olsen J, Troelsen JT: Mapping of HNF4α target genes in intestinal epithelial cells. BMC Gastroenterology 2009, 9(1):68.
9. Vestergaard EM, Troelsen J, Lange A: [Lactose intolerance. Genotyping simplifies and improves diagnosis]. Ugeskr Laeger 2008, 170(42):3312-3314.
10. Schjoldager KT, Maltesen HR, Balmer S, Lund LR, Claesson MH, Sjostrom H, Troelsen JT, Olsen J: Cellular cross talk in the small intestinal mucosa: postnatal lymphocytic immigration elicits a specific epithelial transcriptional response. Am J Physiol Gastrointest Liver Physiol 2008, 294(6):G1335-1343.
11. Enattah NS, Jensen TG, Nielsen M, Lewinski R, Kuokkanen M, Rasinpera H, El-Shanti H, Seo JK, Alifrangis M, Khalil IF et al: Independent Introduction of Two Lactase-Persistence Alleles into Human Populations Reflects Different History of Adaptation to Milk Culture. Am J Hum Genet 2008, 82(1):57-72.
12. Eiberg H, Troelsen J, Nielsen M, Mikkelsen A, Mengel-From J, Kjaer KW, Hansen L: Blue eye color in humans may be caused by a perfectly associated founder mutation in a regulatory element located within the HERC2 gene inhibiting OCA2 expression. Hum Genet 2008, 123(2):177-187.
13. Stegmann A, Hansen M, Wang Y, Larsen JB, Lund LR, Ritie L, Nicholson JK, Quistorff B, Simon-Assmann P, Troelsen JT et al: Metabolome, transcriptome and bioinformatic cis-element analyses point to HNF-4 as a central regulator of gene expression during enterocyte differentiation. Physiol Genomics 2006, 11(2):141-155.
14. Troelsen JT: Adult-type hypolactasia and regulation of lactase expression. Biochimica et Biophysica Acta 2005, 1723(1-3):19-32.
15. Olsen L, Bressendorff S, Troelsen JT, Olsen J: Differentiation-dependent activation of the human intestinal alkaline phosphatase promoter by HNF-4 in intestinal cells. Am J Physiol Gastrointest Liver Physiol 2005, 289(2):G220-G226.
16. Lewinsky RH, Jensen TG, Moller J, Stensballe A, Olsen J, Troelsen JT: T-13910 DNA variant associated with lactase persistence interacts with Oct-1 and stimulates lactase promoter activity in vitro. Hum Mol Genet 2005, 14(24):3945-3953.
17. Olsen L, Hansen M, Ekstrom CT, Troelsen JT, Olsen J: CVD: the intestinal crypt/villus in situ hybridization database. Bioinformatics 2004, 20(8):1327-1328.
18. Troelsen JT, Olsen J, Moller J, Sjostrom H: An upstream polymorphism associated with lactase persistence has increased enhancer activity. Gastroenterology 2003, 125(6):1686-1694.
19. Troelsen JT, Mitchelmore C, Olsen J: An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Gene 2003, 305:101-111.
20. Olsen J, Kirkeby LT, Brorsson MM, Dabelsteen S, Troelsen JT, Bordoy R, Fenger K, Larsson LI, Simon-Assmann P: Converging signals synergistically activate the LAMC2 promoter and lead to accumulation of the laminin gamma 2 chain in human colon carcinoma cells. Biochemical Journal 2003, 371(Pt 1):211-221.
21. Dabelsteen S, Troelsen JT, Olsen J: Identification of keratinocyte proteins that mark subsets of cells in the epidermal stratum basale: comparisons with the intestinal epithelium. OncolRes 2003, 13(6-10):393-398.
22. Tadjali M, Seidelin JB, Olsen J, Troelsen JT: Transcriptome changes during intestinal cell differentiation. Biochimica et Biophysica Acta 2002, 1589(2):160-167.
23. Brimnes MK, Jensen T, Jorgensen TN, Michelsen BK, Troelsen J, Werdelin O: Low expression of insulin in the thymus of non-obese diabetic mice. J Autoimmun 2002, 19(4):203-213.
24. Olsen J, Lefebvre O, Fritsch C, Troelsen JT, Orian-Rousseau V, Kedinger M, Simon-Assmann P: Involvement of activator protein 1 complexes in the epithelium-specific activation of the laminin gamma2-chain gene promoter by hepatocyte growth factor (scatter factor). Biochem J 2000, 347(Pt 2):407-417.
25. Mitchelmore C, Troelsen JT, Spodsberg N, Sjöström H, Norén O: Interaction between the homeodomain proteins Cdx2 and HNF1alpha mediates expression of the lactase-phlorizin hydrolase gene. Biochem J 2000, 346 Pt 2:529-535.
26. Spodsberg N, Troelsen JT, Carlsson P, Enerback S, Sjöström H, Norén O: Transcriptional regulation of pig lactase-phlorizin hydrolase: involvement of HNF-1 and FREACs. Gastroenterology 1999, 116(4):842-854.
27. Mitchelmore C, Troelsen JT, Sjöström H, Norén O: The HOXC11 homeodomain protein interacts with the lactase-phlorizin hydrolase promoter and stimulates HNF1alpha-dependent transcription. Journal of Biological Chemistry 1998, 273(21):13297-13306.
28. Troelsen JT, Mitchelmore C, Spodsberg N, Jensen AM, Noren O, Sjostrom H: Regulation of lactase-phlorizin hydrolase gene expression by the caudal-related homoeodomain protein Cdx-2. Biochem J 1997, 322 ( Pt 3):833-838.
29. Olsen J, Kokholm K, Troelsen JT, Laustsen L: An enhancer with cell-type dependent activity is located between the myeloid and epithelial aminopeptidase N (CD 13) promoters. Biochemical Journal 1997, 322:899-908.
30. Thomsen PD, Johansson M, Troelsen JT, Andersson L: The lactase phlorizin hydrolase (LCT) gene maps to pig chromosome 15q13. Anim Genet 1995, 26(1):49-52.
31. Troelsen JT, Olsen J, Mitchelmore C, Hansen GH, Sjostrom H, Noren O: Two intestinal specific nuclear factors binding to the lactase-phlorizin hydrolase and sucrase-isomaltase promoters are functionally related oligomeric molecules. FEBS Lett 1994, 342(3):297-301.
32. Troelsen JT, Mehlum A, Olsen J, Spodsberg N, Hansen GH, Prydz H, Noren O, Sjostrom H: 1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice. FEBS Lett 1994, 342(3):291-296.
33. Olsen J, Laustsen L, Troelsen J: HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells. FEBS Lett 1994, 342(3):325-328.
34. Torp N, Rossi M, Troelsen JT, Olsen J, Danielsen EM: Lactase-phlorizin hydrolase and aminopeptidase N are differentially regulated in the small intestine of the pig. Biochemical Journal 1993, 295:177-182.
35. Troelsen JT, Olsen J, Noren O, Sjostrom H: A novel intestinal trans-factor (NF-LPH1) interacts with the lactase-phlorizin hydrolase promoter and co-varies with the enzymatic activity. J Biol Chem 1992, 267(28):20407-20411.
Press clippings
CBS news on the discovery of the mutation given blue eyes.
http://www.cbsnews.com/stories/2008/02/07/eveningnews/main3805316.shtml
http://www.cbsnews.com/stories/2008/02/07/eveningnews/main3805316.shtml